The Cytotoxic Effect of Iraqi Rhus Coriaria against Breast and Esophagus Cancer Cells

Abstract

Background:cell lines derived from cancer cells are frequently used in research, including use as a model to understand cancer and to identify potential new treatments. The aim of this study has been prepared to evaluate the anticancer effect of Rhus coriaria that has been studied for their anticancer activity on esophagus and breast cancer cell line. Method: AMJ13(new breast cancer cell line (AMJ13) has been established from an Iraqi breast cancer patient and SK-GT-4 cells (Human esophageal adenocarcinoma cell line) were treated withethyl acetate fraction ofleavesand  fruit parts of plant extractsofRhus coriaria. the MTT assay to determine the anticancer activity was done using 96-well plateswherecell lines were seeded at 1 × 104cells/well after 24 hrs. the cells were treated with tested compounds at different concentrations. Cell viability was measured after 72 hrs of treatment by removing the medium, adding 28 µL of 2 mg/mL solution of MTT, and incubating the cells for 2.5 h at 37 °C. After removing the MTT solution, the crystals remaining in the wells were solubilized by the addition of 130 µL of DMSO (Dimethyl Sulphoxide) followed by 37 °C incubation for 15 min with shaking. The absorbency was determined on a microplate reader at 492 nm. Conclusion: ethyl acetate fraction fromRhus coriaria showed particularly stronganticancercapabilitiessinceitinhibitedactualtumor progression in a breast adenocarcinoma mouse model. Our results suggest thatethyl acetate fraction of plant extracts are promising anticancerreagents that has been studied for their anticancer activity on esophagus and breast cancer cell line.