Cuminaldehyde reduces diethylhexyl phthalate-induced insulin resistance in human Chang liver cells

Abstract

(DEHP) exposed Chang liver cells in vitro. Methods: Chang liver cells (5x10⁵ cells) were exposed to 400µM of DEHP for 24h. At the end of incubation, cells were treated with different doses of (50, 100 and 200 µg/ml) cuminaldehyde for 48 h. After 48h, the cells were used for the assessment of insulin signalling molecules (IR, IRS-2, and Akt) and glucose oxidation in Chang liver cells. Results: IR, IRS-2 and Akt mRNA and glucose oxidation in DEHP-exposed Chang liver cells were significantly reduced (p<0.05). Treatment with cuminaldehyde enhanced IR, IRS-2 and Akt mRNA and oxidation of glucose significantly (p<0.05). Conclusion: The present findings are the first to report to identify the antidiabetic role of cuminaldehyde against DEHP exposed detrimental changes insulin signalling molecules and glucose oxidation in liver cells. Hence, cuminaldehyde may have significant role in controlling diabetes and hence it could be used a therapeutic natural drug for the treatment of diabetes mellitus.