Isolation and Structural Elucidation of Linolenic Acid (Omega-3-Polyunsaturated Fatty Acid) from Whole Plant of Petiveria alliacea L.

Abstract

The whole plant of Petiveria alliacea (Phytolaccaceae) was isolated and subjected to structural elucidation of chemical constituent. The petroleum ether fraction was purified and isolated using column chromatography and thin layer chromatography. Then the isolated compound was subjected to FTIR for recognition of functional groups and ¹HNMR and ¹³CNMR for position of protons and carbon atoms are also subjected to  UV-spectrum. ESI-MS was done to recognize the molecular weight of the isolated compound. From FT-IR, ¹HNMR, ¹³CNMR and ESI-MS, the isolated compound was found to be linolenic acid (i.e. omega-3-polyunsaturated fatty acid) and this is obtained from the spectra found.