Antioxidant Supplementation Reduces Oxidative Stress in Doxorubicin Treated Female Rats

Abstract

Infertility affects approximately 13% of women and 10% of men. The major causes of female infertility are anovulation, fallopian tube disease, endometriosis, and chemotherapy, Cases treated with doxorubicin  increases the release of ROS that attack and damage energetic molecules in the cell.female reproductive system is particularly vulnerable to ROS because of inherent deficiencies in intracellular antioxidant . Antioxidants are compounds at act on oppose formation of the actions ROS ,thus total antioxidant capacity become more substantial to protect cells. in this study was to estimate the ability of different antioxidants in the protection of  female fertility through reducing oxidative stress in oxidative stress induced female reproductive dysfunction .

In this study to try to evaluate the role of serum total antioxidant capacity, glutathione peroxidase, catalase, superoxide dismutase, and malondialdehyde  in female rats infertility.Forty  female rats (aged of 3-4 months and weight of 300-400 gm) were divided into 5 groups including, group1 as a control group which received drinking normal salineorally, group2 treated with doxorubicin at concentration 15mg/kgsubcutaneous injection. group 3, treated with curcumin  at 200 mg/kgorally, group 4 , treated with melatonin at 15 mg/kgorally, group 5  treated with Vitamin E at 200 mg/kg orally. Result showed that doxorubicin treatment revealed a significant increase  in (GPx) levels in the serum for the all of the treatment groups compared with control group . While There was no significant difference in the level of  CAT , TACand MDAin compare with control, the results indicated for a significant increase in SOD serum levels during  doxorubicin treatment in melatonin, Vitamin E and  decrease in activity of SODinCurcumin.

In conclusion , using antioxidants are useful in prevention of female fertility during chemotherapy treatment by doxorubicin .