Drugs and Cell Therapies in Hematology

Additive Inverse Cordial Labeling Of Some Derived Graphs

2022-05-20T08:24:56+00:00

Additive Inverse Cordial Labeling Of Some Derived Graphs

Prevalence Of HDV Infection in Sindh Pakistan: A Molecular Approach diagnosed at LUMHS Hyderabad

2022-06-06T15:51:57+00:00

Introduction: Hepatitis Delta Virus (HDV) infects only patients that are already infected by hepatitis B virus (HBV) because this is sub satellite virus which depends on and propagate only in the presence of HBV. HDV causes co-infection or super infection with sever complication as compared to only HBV infection. No study on molecular level on HDV is available from this region.

Purpose/Objectives: The aim of this study was to find out the molecular epidemiology of HDV (as a co-infection with HBV) in different geographical regions of Sindh.

Study Design: Cross Sectional Descriptive Study.

Place And Duration of Study: It was carried out at Diagnostic & Research Laboratory LUMHS Hyderabad from June 2017 to December 2018.

Material And Methods/Results: Whole Blood samples were collected in 5 ml vacutainer. Total 1069 HBsAg positive samples were received for the study from different geographical regions of the sindh. Only HBV DNA PCR positive samples were further utilized for the presence of HDV RNA. For this purpose, HDV RNA and HBV DNA was extracted and amplified using reverse transcriptase polymerase chain reaction (RT-PCR), for HDV extraction was done using Roche high pure vial nucleic acid kit & amplication was done using light cycler mix kit (Roche) on instrument z480 (Roche). Out of the total 1069 HBsAg positive samples, HBD was detected in total 562 (52.50%) samples belonged to different patients. From 773 males 415 (38.8%) were positive while from 296 females 147 (13.7%) were positive. The percentage of dual infection was found higher in male patients as compared to female patients. KandhKot, KandiaroGhotki, PanoAqil and KhairpurMirsobserved higher prevalence of HDV i-e 67%, 66%, 57%, 55% and also 55%respectively as compared to other cities in the province of Sindh.

Conclusion: The HDV infection is not uncommon in Sindh. The only way to prevent the infection is by educating the general population through a planned prevention program.

A Study to Check the Antibacterial Activity of Herbal Powder Extracts against Klebsiella Pneumonia

2022-06-07T10:23:02+00:00

Aim: This study wasconducted to discover the newcompounds from the extracts of medicinal herbs. The goal of the present research was to investigate the bactericidalactivity of herbal powder extracts.

Method: In this study, the agar well diffusion method was selected to check the antibacterial of herbal powder extracts of Finger millet (Eleusinecoracana) and Sandal (Santalum album). Agar well diffusion method was used to checkantibacterial activity against six pathogens of Gram-positive which include Bacillus, Methicillin-resistant Staphylococcus aureus (MRSA), Klebsiella pneumonia (K.pneumon), Pseudomonas aeruginosa andEscherichia coli (E.coli).

Result: From this research, it was revealed that both extracts of Finger millet and Sandal showed antibacterial activity against (K.pneumon).

Conclusion: In conclusion, both herbal extracts were found to be significantly effective. Further research is needed tobe done for confirmation.

Prevalence of HDV Infection in Sindh Pakistan: A Molecular Approach Diagnosed at LUMHS Hyderabad

2022-06-10T09:51:06+00:00

INTRODUCTION: Hepatitis Delta Virus (HDV) infects only patients that are already infected by hepatitis B virus (HBV) because this is sub satellite virus which depends on and propagate only in the presence of HBV. HDV causes co-infection or super infection with sever complication as compared to only HBV infection. No study on molecular level on HDV is available from this region.

PURPOSE/OBJECTIVES: The aim of this study was to find out the molecular epidemiology of HDV (as a co-infection with HBV) in different geographical regions of Sindh.

STUDY DESIGN: Cross Sectional Descriptive Study.

PLACE AND DURATION OF STUDY: It was carried out at Diagnostic & Research Laboratory LUMHS Hyderabad from June 2017 to December 2018.

MATERIAL AND METHODS/RESULTS: Whole Blood samples were collected in 5 ml vacutainer.Total 1069 HBsAg positive samples were received for the study from different geographical regions of the sindh. Only HBV DNA PCR positive samples were further utilized for the presence of HDV RNA. For this purpose, HDV RNA and HBV DNA was extracted and amplified using reverse transcriptase polymerase chain reaction (RT-PCR), for HDV extraction was done using Roche high pure vial nucleic acid kit & amplication was done using light cycler mix kit (Roche) on instrument z480 (Roche). Out of the total 1069 HBsAg positive samples, HBD was detected in total 562 (52.50%) samples belonged to different patients. From 773 males 415 (38.8%) were positive while from 296 females 147 (13.7%) were positive. The percentage of dual infection was found higher in male patients as compared to female patients. KandhKot, KandiaroGhotki, PanoAqil and KhairpurMirsobserved higher prevalence of HDV i-e 67%, 66%, 57%, 55% and also 55%respectively as compared to other cities in the province of Sindh.

CONCLUSION: The HDV infection is not uncommon in Sindh. The only way to prevent the infection is by educating the general population through a planned prevention program.

Evaluation of Mechanical Properties of Polypropylene with Molybdenum Disulphide

2022-08-06T10:43:05+00:00

The presented investigation results confirm purposefulness of employing injection moulding and extrusion for fabrication of blends of polypropylene (PP) and Molybdenum disulfide (MoS₂) polymer matrix to generate novel composite materials through an advantageous melt-processing route. These two methods are used for manufacturing elements of devices in a mass scale.

The effect of different composition of MoS₂ on Polypropylene material performance was studied on samples which were directly obtained from extrusion and on injection moulding. Tensile, Compression, Flexural, Izod impact, micro hardness, Scanning electron microscope (SEM) tests were conducted on the test samples containing different composition of MoS₂ varying from 0.93 to 7.12 vol.%. It was found that there was a homogenous distribution of the Molybdenum disulphide (MoS₂) particles in polypropylene (PP) and a good bonding was observed between them. The incorporation of MoS₂ generates notable performance enhancements through reinforcement effects, highly efficient nucleation activity and good bonding ability.

However, the mechanical properties of the composites containing above 4.28 vol.%MoS₂ was deteriorated, as compared to that of composites containing lower MoS₂ content.

Community Partication and Challenges

2022-08-06T10:47:12+00:00

The community participation is very important towards the development of health care in the community which helps in solving health issues that derails the community, the community participation and challenges have resulted in maladministration, fraud and corruption which culminatesin failure to deliver effective and efficient services. This impacts negatively on the lives of the citizenry because they are forced to live in untenable conditions due to poor service delivery. an independent community office or body should be established to enforce community participation and members of the council be coerced to consult and account to their communities of the circumstances in the municipality. this could enhance transparency and accountability among municipalities and provide an impetus in service delivery. Consequently, public participation could be revitalised in rural and urban areas where community participation is neglected.