Chronic Toxicity Study of Poly Herbal Formulation (PHF) in Albino Wistar Rats


  • Dr.Subasini Uthirapathy


In recent years, there has been a greater focus on the possible toxicity of several herbal medicines. Many different forms of poly herbal formulations are utilized in ancient systems of medicine such as Ayurveda, Siddha, and Homoeopathy. Many herbal formulations have been shown in animal models and clinical trials to be an effective agent for treating coronary heart disease, peptic ulcers, and skin problems etc., however, nothing is known regarding PHF's potential chronic toxicity. The purpose of this study was to explore at the safety of poly herbal formulation (PHF) in laboratory rats. Wistar rats were separated into four groups of 50 rats each for chronic toxicity (20 males and 20 female). The experimental animals were administered PHF at doses of 250,500, and 1000mg/kg for 180 days, satellite group animals also treated with 1000 mg/kg. while the control animals were given distilled water. The animal's body weight, food and water intake were all tracked on a weekly basis. In addition, at certain observation time points, their biochemical and haematological parameters, histology, and body and organ weights were also measured. PHF did not induce noticeable anomalies in physiological parameters or pathological changes in the major organs of rats, according to the results of the chronic toxicity study. The results showed that PHF at the specified levels had no adverse effects on the animals. As a result, it is acceptable to conclude that PHF is safe at the dosage levels studied and does not produce chronic toxicity in animal models.




How to Cite

Dr.Subasini Uthirapathy. (2021). Chronic Toxicity Study of Poly Herbal Formulation (PHF) in Albino Wistar Rats . Drugs and Cell Therapies in Hematology, 10(3), 343–359. Retrieved from